The process of sexing semen is precise and involves the following steps:

DNA Staining: The sperm are treated with a DNA-binding fluorescent dye, which attaches to theDNA, allowing sperm to be visualized and sorted.

Flow Cytometry: The sperm pass through a flow cytometer that analyzes each sperm cell as it passes through a laser beam. The fluorescence emitted by the stained sperm helps determine whether the sperm is
carrying an X or Y chromosome.

Sorting: The flow cytometer sorts the sperm into two groups:

• X-Linked Sperm (Female): These sperm contain more DNA and fluoresce brightly.
• Y-Linked Sperm (Male): These sperm contain less DNA and fluoresce dimly.

This sorting process ensures a 95%accuracy rate for selecting the desired sex of the foal, though it’s important to note that it is not 100% guaranteed.

Gender Selection: Sexed semen allows you to select the foal’s sex, which is especially valuable for performance-focused breeding. For example, breeders may prefer female foals for breeding programs or male foals for competition.

Enhanced Breeding Programs: This technology can improve the efficiency and success of breeding programs, particularly for high-value mares and stallions where producing foals of a specific sex is highly desirable.

Precision in Selective Breeding: By controlling foal gender, breeders can more effectively plan and manage breeding strategies, targeting specific disciplines or desirable traits.

Utilizing sexed semen helps breeders achieve their goals faster while advancing the genetic quality of their herd. This technology enables breeders to:

Mitigate Gender-Specific Genetic Diseases: Some genetic disorders are gender-linked, and sexed semen allows breeders to avoid passing on these conditions.

Optimize Breeding Outcomes: By selecting the optimal gender, breeders can achieve the highest potential for success in their breeding programs